Cloning and expression analysis of the Ccrboh gene encoding respiratory burst oxidase in Citrullus colocynthis and grafting onto Citrullus lanatus (watermelon)

Cloning and expression analysis of the Ccrboh gene encoding respiratory burst oxidase in Citrullus colocynthis and grafting onto Citrullus lanatus (watermelon)

A full-length drought-responsive gene Ccrboh, encoding the respiratory burst oxidase homologue (rboh), was cloned in Citrullus colocynthis, a very drought-tolerant cucurbit species. The robh protein, also named NADPH oxidase, is conserved in plants and animals, and functions in the production of reactive oxygen species (ROS). The Ccrboh gene accumulated in a tissue-specific pattern when C. colo...

Molecular cloning, sequence characterization and expression pattern of Rab18 gene from watermelon (Citrullus lanatus)

The complete mRNA sequence of watermelon Rab18 gene was amplified through the rapid amplification of cDNA ends (RACE) method. The full-length mRNA was 1010 bp containing a 645 bp open reading frame, which encodes a protein of 214 amino acids. Sequence analysis revealed that watermelon Rab18 protein shares high homology with the Rab18 of cucumber (99%), muskmelon (98%), Morus notabilis (90%), to...

Origin and emergence of the sweet dessert watermelon, Citrullus lanatus.

BACKGROUND AND AIMS Watermelons, Citrullus species (Cucurbitaceae), are native to Africa and have been cultivated since ancient times. The fruit flesh of wild watermelons is watery, but typically hard-textured, pale-coloured and bland or bitter. The familiar sweet dessert watermelons, C. lanatus, featuring non-bitter, tender, well-coloured flesh, have a narrow genetic base, suggesting that they...

Lycopene and Citrulline Contents in Watermelon (Citrullus lanatus)

Lycopene and citrulline contents of 9 diploids (2X) and their artificially induced autotetraploids (4X) and autotriploids (3X) were determined in mature fruit. Changes in lycopene and citrulline during fruit development was studied using fruit of variety XYXA with three different ploidy levels from 15 to 40 days after pollination (DAP). Polyploids, especially triploids, had higher levels of lyc...

Recombinant Watermelon (Citrullus Lanatus) Hydroperoxide Lyase and Uses Thereof